Technology Platform

Bio-Materials
Cellular Engineering
Exosome & Nanotherapy
Bio-Fabrication
Bio-engineered Extra Cellular Matrix mimic for tissue regeneration and scar-less wound healing

Bio-Materials

The Biomaterial vertical aims to develop a range of biomaterials that enable control of the extracellular environment of target tissues, that interact with cellular components and provide a bio-mimetic environment that is able to instruct the cells within to mirror native tissue like functions. In this regard, we have developed a cornea specific proprietary “Bio-Ink” that has a native tissue like transparency, and had has similar mechanical and chemical properties. Our teams smartly use the combined properties of bio-mimetic elements with directed design properties, such as tunable adhesiveness and biodegradation, to push for new avenues in tissue engineering that can develop new products for unmet needs of patients.
Scalable 2D and 3D Mesechymal Stem Cell (MSC) culture for robust and scalable production of cells and derived therapeutics

Cellular Engineering

Cellular engineering (and propietary Cell farming) vertical of Pandorum essentially deals with the expansion of stem cells form different tissue sources in a robust and scalable manner. Pandorum’s expertise to culture cells in 2D or 3D environment for optimized cellular expression for production of cell and cell derived components with therapeutic potential is key to various product formulation with regenerative capacities.

Regenerative Cell Modulators - MSC derived exosomes at high purity with multi-modal characterization

Exosome and Nanotherapy

Exosomes are cell secreted lipid nanovescicles of the size range of ~30–150 nm, containing various cargos with pleiotropic functions including protein, mRNA and miRNA molecules, for cell-to-cell communication. Using the inherent plasticity of the stem cells combined with cell-farming technology and proprietary culture methods, we have been able to optimize the cell expression profile in a way that the cells secrete therapeutically enriched exosomes. Detailed characterization of various exosomal cargos with functional assay have allowed us to screen and optimize production of exosomes with superior anti-inflammatory, anti-fibrotic and cell migration properties. The exosomes are specifically developed to promote accelerated regeneration of specific tissue targets, including avascular tissue (such as, cornea), and vascular tissue (such as, Lung, Liver). The tissue agnostic platform core allows Pandorum to develop advanced regenerative therapeutics optimized for various targets/ indications- including lung, cornea and beyond.

Video-microscopy data captured in Pandorum’s lab (MUSC) shows cellular level events leading to wound healing, using Exosome stimulated migration of Corneal Epithelial Cells, covering the zone of separation ~300 micron (marked by the dotted line) created in a scratch assay. The arrow at different time points shows a small ‘pillar’ of leader cells- first ones to bridging the gap

4D Bioprinting approach

Bio-fabrication

Bioprinting is the hierarchical assembly of 3D biological structures for tissue construction. Pandorum leverages this state of the art extrusion-based bioprinter to mimic microenvironment and architecture of human tissues. “Time”, when integrated as the fourth dimension, the printed structure dynamically changes its form to express tissue-specific functionalities. Pandorum combines various fabrication processes in its product development pipelines to ‘build the blocks of life’.